Anti-Tuberculosis Drugs Co-Administration Aggravates the Testicular Toxicity of Ethanol in Rats

Alcohol is well documented to affect male fertility. The association between alcohol use disorders and tuberculosis has long been known and well described. Taking into account that both alcohol and anti-tuberculosis drugs (ATD) could have negative impacts on male gonads, an increase in adverse effects is probable. The study aimed to determine combine effects of long-term alcohol consumption and ATD administration on male rat testes CYP2E1 mRNA and protein expression, DNA fragmentation, spermatogenesis parameters, as well as on male reproductive capacity and antenatal development of its posterity. Wistar albino male rats were divided into three groups: I – control (intact animals), II – chronic alcoholism (15% ethanol selfadministration during 150 days): IIIchronic alcoholism + ATD (ethambutol, isoniazid, rifampin and pyrazinamide) administration. ombined effects of chronic alcoholism and ATD led to changes in testicular CYP2E1 mRNA and protein expression, as well as in DNA fragmentation processes. These abnormalities were more expressed in alcoholic with ATD co-administration group as compared with alcoholic group. The revealed disorders at levels of testicular genome and proteome caused negative implications on spermatogenic epithelium quantitative and qualitative parameters, sperm count, male fertility, and postimplantational surviving of its offspring. Thus the ATD administration modulated the impact of long-term alcohol consumption on spermatogenesis and aggravated paternal-mediated negative effects on posterity.